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Silver staining of electrophoresis gels and Western blots on organic matrix extracted from sclerites of C. rubrum . a , one-dimensional SDS-PAGE (10 μg protein/lane; BisTris 12% polyacrylamide gel, silver stain molecular mass marker (M6539; Sigma) as protein marker). b , one-dimensional SDS-PAGE (10 μg protein/lane, Tris-Tricine 16.5% polyacrylamide gel, <t>kaleidoscope</t> <t>polypeptide</t> standards (161-0325; Bio-Rad) as protein marker); c , two-dimensional electrophoresis gel (Tris-Tricine 16.5% polyacrylamide gel, kaleidoscope polypeptide standards (161-0325) as protein marker) of the major protein band (18*) excised from one dimensional SDS-PAGE. d , Western blot (5 μg of proteins/lane; BisTris 12% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376; Bio-Rad) as protein marker) with antibodies against phosphorylated serine. e , Western blot with antibodies against the phosphorylated threonine (5 μg of proteins/lane; BisTris 12% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376) as protein marker). f , Western blot with antibodies against phosphorylated tyrosine (5 μg of proteins/lane; BisTris 12% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376) as protein marker). g , Western blot with anti-scleritin antibodies (Tris-Tricine 16.5% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376) as protein marker). MW , molecular mass; M , protein marker; and triangles show apparent molecular mass of proteins (in kDa).
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Silver staining of electrophoresis gels and Western blots on organic matrix extracted from sclerites of C. rubrum . a , one-dimensional SDS-PAGE (10 μg protein/lane; BisTris 12% polyacrylamide gel, silver stain molecular mass marker (M6539; Sigma) as protein marker). b , one-dimensional SDS-PAGE (10 μg protein/lane, Tris-Tricine 16.5% polyacrylamide gel, <t>kaleidoscope</t> <t>polypeptide</t> standards (161-0325; Bio-Rad) as protein marker); c , two-dimensional electrophoresis gel (Tris-Tricine 16.5% polyacrylamide gel, kaleidoscope polypeptide standards (161-0325) as protein marker) of the major protein band (18*) excised from one dimensional SDS-PAGE. d , Western blot (5 μg of proteins/lane; BisTris 12% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376; Bio-Rad) as protein marker) with antibodies against phosphorylated serine. e , Western blot with antibodies against the phosphorylated threonine (5 μg of proteins/lane; BisTris 12% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376) as protein marker). f , Western blot with antibodies against phosphorylated tyrosine (5 μg of proteins/lane; BisTris 12% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376) as protein marker). g , Western blot with anti-scleritin antibodies (Tris-Tricine 16.5% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376) as protein marker). MW , molecular mass; M , protein marker; and triangles show apparent molecular mass of proteins (in kDa).
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Silver staining of electrophoresis gels and Western blots on organic matrix extracted from sclerites of C. rubrum . a , one-dimensional SDS-PAGE (10 μg protein/lane; BisTris 12% polyacrylamide gel, silver stain molecular mass marker (M6539; Sigma) as protein marker). b , one-dimensional SDS-PAGE (10 μg protein/lane, Tris-Tricine 16.5% polyacrylamide gel, <t>kaleidoscope</t> <t>polypeptide</t> standards (161-0325; Bio-Rad) as protein marker); c , two-dimensional electrophoresis gel (Tris-Tricine 16.5% polyacrylamide gel, kaleidoscope polypeptide standards (161-0325) as protein marker) of the major protein band (18*) excised from one dimensional SDS-PAGE. d , Western blot (5 μg of proteins/lane; BisTris 12% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376; Bio-Rad) as protein marker) with antibodies against phosphorylated serine. e , Western blot with antibodies against the phosphorylated threonine (5 μg of proteins/lane; BisTris 12% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376) as protein marker). f , Western blot with antibodies against phosphorylated tyrosine (5 μg of proteins/lane; BisTris 12% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376) as protein marker). g , Western blot with anti-scleritin antibodies (Tris-Tricine 16.5% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376) as protein marker). MW , molecular mass; M , protein marker; and triangles show apparent molecular mass of proteins (in kDa).
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Silver staining of electrophoresis gels and Western blots on organic matrix extracted from sclerites of C. rubrum . a , one-dimensional SDS-PAGE (10 μg protein/lane; BisTris 12% polyacrylamide gel, silver stain molecular mass marker (M6539; Sigma) as protein marker). b , one-dimensional SDS-PAGE (10 μg protein/lane, Tris-Tricine 16.5% polyacrylamide gel, <t>kaleidoscope</t> <t>polypeptide</t> standards (161-0325; Bio-Rad) as protein marker); c , two-dimensional electrophoresis gel (Tris-Tricine 16.5% polyacrylamide gel, kaleidoscope polypeptide standards (161-0325) as protein marker) of the major protein band (18*) excised from one dimensional SDS-PAGE. d , Western blot (5 μg of proteins/lane; BisTris 12% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376; Bio-Rad) as protein marker) with antibodies against phosphorylated serine. e , Western blot with antibodies against the phosphorylated threonine (5 μg of proteins/lane; BisTris 12% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376) as protein marker). f , Western blot with antibodies against phosphorylated tyrosine (5 μg of proteins/lane; BisTris 12% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376) as protein marker). g , Western blot with anti-scleritin antibodies (Tris-Tricine 16.5% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376) as protein marker). MW , molecular mass; M , protein marker; and triangles show apparent molecular mass of proteins (in kDa).
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Silver staining of electrophoresis gels and Western blots on organic matrix extracted from sclerites of C. rubrum . a , one-dimensional SDS-PAGE (10 μg protein/lane; BisTris 12% polyacrylamide gel, silver stain molecular mass marker (M6539; Sigma) as protein marker). b , one-dimensional SDS-PAGE (10 μg protein/lane, Tris-Tricine 16.5% polyacrylamide gel, <t>kaleidoscope</t> <t>polypeptide</t> standards (161-0325; Bio-Rad) as protein marker); c , two-dimensional electrophoresis gel (Tris-Tricine 16.5% polyacrylamide gel, kaleidoscope polypeptide standards (161-0325) as protein marker) of the major protein band (18*) excised from one dimensional SDS-PAGE. d , Western blot (5 μg of proteins/lane; BisTris 12% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376; Bio-Rad) as protein marker) with antibodies against phosphorylated serine. e , Western blot with antibodies against the phosphorylated threonine (5 μg of proteins/lane; BisTris 12% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376) as protein marker). f , Western blot with antibodies against phosphorylated tyrosine (5 μg of proteins/lane; BisTris 12% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376) as protein marker). g , Western blot with anti-scleritin antibodies (Tris-Tricine 16.5% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376) as protein marker). MW , molecular mass; M , protein marker; and triangles show apparent molecular mass of proteins (in kDa).
Molecular Weight Marker Precision Plus Protein Kaleidoscope, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad precision plus protein standard kaleidoscop molecular weight marker
Silver staining of electrophoresis gels and Western blots on organic matrix extracted from sclerites of C. rubrum . a , one-dimensional SDS-PAGE (10 μg protein/lane; BisTris 12% polyacrylamide gel, silver stain molecular mass marker (M6539; Sigma) as protein marker). b , one-dimensional SDS-PAGE (10 μg protein/lane, Tris-Tricine 16.5% polyacrylamide gel, <t>kaleidoscope</t> <t>polypeptide</t> standards (161-0325; Bio-Rad) as protein marker); c , two-dimensional electrophoresis gel (Tris-Tricine 16.5% polyacrylamide gel, kaleidoscope polypeptide standards (161-0325) as protein marker) of the major protein band (18*) excised from one dimensional SDS-PAGE. d , Western blot (5 μg of proteins/lane; BisTris 12% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376; Bio-Rad) as protein marker) with antibodies against phosphorylated serine. e , Western blot with antibodies against the phosphorylated threonine (5 μg of proteins/lane; BisTris 12% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376) as protein marker). f , Western blot with antibodies against phosphorylated tyrosine (5 μg of proteins/lane; BisTris 12% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376) as protein marker). g , Western blot with anti-scleritin antibodies (Tris-Tricine 16.5% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376) as protein marker). MW , molecular mass; M , protein marker; and triangles show apparent molecular mass of proteins (in kDa).
Precision Plus Protein Standard Kaleidoscop Molecular Weight Marker, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad molecular weight marker
Silver staining of electrophoresis gels and Western blots on organic matrix extracted from sclerites of C. rubrum . a , one-dimensional SDS-PAGE (10 μg protein/lane; BisTris 12% polyacrylamide gel, silver stain molecular mass marker (M6539; Sigma) as protein marker). b , one-dimensional SDS-PAGE (10 μg protein/lane, Tris-Tricine 16.5% polyacrylamide gel, <t>kaleidoscope</t> <t>polypeptide</t> standards (161-0325; Bio-Rad) as protein marker); c , two-dimensional electrophoresis gel (Tris-Tricine 16.5% polyacrylamide gel, kaleidoscope polypeptide standards (161-0325) as protein marker) of the major protein band (18*) excised from one dimensional SDS-PAGE. d , Western blot (5 μg of proteins/lane; BisTris 12% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376; Bio-Rad) as protein marker) with antibodies against phosphorylated serine. e , Western blot with antibodies against the phosphorylated threonine (5 μg of proteins/lane; BisTris 12% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376) as protein marker). f , Western blot with antibodies against phosphorylated tyrosine (5 μg of proteins/lane; BisTris 12% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376) as protein marker). g , Western blot with anti-scleritin antibodies (Tris-Tricine 16.5% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376) as protein marker). MW , molecular mass; M , protein marker; and triangles show apparent molecular mass of proteins (in kDa).
Molecular Weight Marker, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Silver staining of electrophoresis gels and Western blots on organic matrix extracted from sclerites of C. rubrum . a , one-dimensional SDS-PAGE (10 μg protein/lane; BisTris 12% polyacrylamide gel, silver stain molecular mass marker (M6539; Sigma) as protein marker). b , one-dimensional SDS-PAGE (10 μg protein/lane, Tris-Tricine 16.5% polyacrylamide gel, kaleidoscope polypeptide standards (161-0325; Bio-Rad) as protein marker); c , two-dimensional electrophoresis gel (Tris-Tricine 16.5% polyacrylamide gel, kaleidoscope polypeptide standards (161-0325) as protein marker) of the major protein band (18*) excised from one dimensional SDS-PAGE. d , Western blot (5 μg of proteins/lane; BisTris 12% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376; Bio-Rad) as protein marker) with antibodies against phosphorylated serine. e , Western blot with antibodies against the phosphorylated threonine (5 μg of proteins/lane; BisTris 12% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376) as protein marker). f , Western blot with antibodies against phosphorylated tyrosine (5 μg of proteins/lane; BisTris 12% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376) as protein marker). g , Western blot with anti-scleritin antibodies (Tris-Tricine 16.5% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376) as protein marker). MW , molecular mass; M , protein marker; and triangles show apparent molecular mass of proteins (in kDa).

Journal: The Journal of Biological Chemistry

Article Title: Molecular Cloning and Characterization of First Organic Matrix Protein from Sclerites of Red Coral, Corallium rubrum *

doi: 10.1074/jbc.M112.352005

Figure Lengend Snippet: Silver staining of electrophoresis gels and Western blots on organic matrix extracted from sclerites of C. rubrum . a , one-dimensional SDS-PAGE (10 μg protein/lane; BisTris 12% polyacrylamide gel, silver stain molecular mass marker (M6539; Sigma) as protein marker). b , one-dimensional SDS-PAGE (10 μg protein/lane, Tris-Tricine 16.5% polyacrylamide gel, kaleidoscope polypeptide standards (161-0325; Bio-Rad) as protein marker); c , two-dimensional electrophoresis gel (Tris-Tricine 16.5% polyacrylamide gel, kaleidoscope polypeptide standards (161-0325) as protein marker) of the major protein band (18*) excised from one dimensional SDS-PAGE. d , Western blot (5 μg of proteins/lane; BisTris 12% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376; Bio-Rad) as protein marker) with antibodies against phosphorylated serine. e , Western blot with antibodies against the phosphorylated threonine (5 μg of proteins/lane; BisTris 12% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376) as protein marker). f , Western blot with antibodies against phosphorylated tyrosine (5 μg of proteins/lane; BisTris 12% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376) as protein marker). g , Western blot with anti-scleritin antibodies (Tris-Tricine 16.5% polyacrylamide gel, Precision Plus Protein WesternC TM standard (161-0376) as protein marker). MW , molecular mass; M , protein marker; and triangles show apparent molecular mass of proteins (in kDa).

Article Snippet: The protein markers used were: Silver Stain Molecular Weight Marker (M6539; Sigma) for the BisTris silver-stained electrophoresis gel, Kaleidoscope Polypeptide Standards (Bio-Rad 161-0325) for the Tris-Tricine silver-stained electrophoresis gels, and Precision Plus Protein WesternC TM standard (161-0376 Bio-Rad) both for the BisTris and Tris-Tricine gels of Western blots.

Techniques: Silver Staining, Electrophoresis, Western Blot, SDS Page, Marker